Immobilization of the Amidohydrolase MxcM and Its Application for Biocatalytic Flow Synthesis of Pseudochelin A

The chemical synthesis of heterocycles typically requires elevated temperature and acid or base addition to form the desired product. Moreover, these reactions often involve hazardous reagents, which is why biocatalytic routes for heterocycle formation have gained increasing attention. In recent years, several enzymes belonging amidohydrolase superfamily been identified generate via cyclocondensation reactions. Of particular interest MxcM, catalyzes an imidazoline moiety in biosynthesis anti-inflammatory natural product pseudochelin A. this study, we present a concept immobilization enzyme using fused hexahistidine tag fixation onto solid, porous carrier. Notably, improves enzyme’s tolerance organic solvents. immobilized MxcM exhibits residual activity 169% polar solvent acetonitrile compared free enzyme, storage stability presence 20 vol% was ameliorated. addition, reactor (IMER) designed that can be operated under flow conditions. MxcM-IMER retains its mechanic over tested operation time. These results provide important insights integration heterocycle-forming amidohydrolases processes.